Transcriptome analysis in treated MCF7-V breast carcinoma cell line stably expressing a doxycyline-inducible construct to express ZEB1.

The epithelial to mesenchymal transition (EMT) is implicated in the metastatic spread of breast cancer cells. EMT transcription factors (TF) regulate different stages of EMT states. In breast cancers, estrogen receptor a (ERa) maintains the epithelial characteristics of breast tumors and is indispensable for efficient endocrine therapy. In this study we investigate whether and how ZEB1, an EMT-TF affects ERa signaling at early stages of EMT and metastasis. In MCF7-V cells, we used RNA-seq to study the impact of ZEB1 on the ERa transcriptome in breast cancer cells induced with DMSO, 17-beta estradiol (E2), and forskolin + 3-isobutyl-1-methylxanthine (IBMX) (FI). Overall design: We did RNA-seq in wild type MCF7-V breast cancer cells and in cells with a doxycyline-inducible construct to express ZEB1 in presence of dxycycline. We induced ERa activation by DMSO, 17-beta estradiol (E2), and Forskolin + 3-isobutyl-1-methylxanthine (IBMX) (FI). To induce ZEB1 in stably expressing doxycyline-inducible constrcut to express ZEB1, we used 2 µg/ml doxycyline (DOX). nDOX stands no doxycycline.