Derivation of kidney organoids from human pluripotent stem cells [RNA-Seq: Data Set 2]
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=d958f47391443f112e9cc6a4cc35b7a9
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资源简介：
We have developed a robust methodology for the derivation of kidney organoids from human pluripotent stem cells further vascularized taking advantage of several in vivo approaches. Our methodology suffices for the generation of kidney derivatives transcriptomically comparable with 22 weeks of gestation kidney tissues and highlight the use of longer periods of exposure to 3D in order to promote renal differentiation in a time frame of 15 days. In order to ascertain the impact of extracellular matrix microenvironment in renal differentiation undifferentiated human pluripotent stem cells were further cultured in compliant substrates mirroring embryo-ECM microenvironment (1kPa) in order to promote renal differentiation and compared these conditions in front of rigid substrates (60 kPa) mimicking hard substrates normally used when differentiating human pluripotent stem cells
提供机构：
Institute for Bioengineering of Catalonia (IBEC)
创建时间：
2022-02-20
