The effect of Anaerostipes caccaei and Bifidobacterium bifidum on epithelial cells of colon

The purpose of this study is to determine whether cross feeding relationships can contribute to the alleviation of colitis. The two strains used in this study are Anaerostipes caccae and Bifidobacterium bifidum. Both strains are clearly present in significant amounts in the intestines of healthy humans. A. caccae is a butyrate-producing bacterium that uses lactic acid and acetate to produce butyrate. On the other hand, B. bifidum is known to produce lactic acid and acetate. The purpose of this study is to confirm the effect of the ecological network between the two strains on the improvement of colitis, particularly in terms of protecting intestinal epithelial cells. Overall design: RNA-seq profiling of colonic epilthelial cells of DSS-induced murine colitis mouse with A. caccae and B. bifidum treatment