Expression data of LX2 transfected with Adenoviruses overexpressing Gata4 or GFP. Expression data of LX2 transfected with Adenoviruses overexpressing Gata4 or GFP

Deactivation of aHSCs has emerged as a novel and promising therapeutic approach for liver fibrosis. However, our knowledge of the master regulators involved in the deactivation of fibrotic HSCs is still limited. The transcription factor GATA4 has been previously shown to play an important role in embryonic hepatic stellate cells quiescence. We aim to evaluate whether GATA4 is able to revert the active phenotype of LX2 cells. Adenoviruses approaches were used to genetically manipulate Gata4 expression in adult hepatic stellate cells. To analyze changes in gene expression mediated by GATA4 we overexpressed Gata4 in LX2 cells and performed Affimetrix analyses Overall design: 2 samples per triplicate. GFP as control and G4 as experimental. LX2 were transfected with each adenoviruses at MOI100 and RNA was collected after 72h of transfection