The Sound Life Project Healthy Adult Immunotypes

Understanding the variability of immune cell composition and responsiveness in health is critical to define changes that predict and explain immune-associated diseases like autoimmunity and cancer. Here, we enrolled and comprehensively phenotyped a cohort of 100 healthy adults aged 25 to 35 and 55 to 65 years who were longitudinally followed for 10 visits over 2 years. Using mass cytometry features that remain stable within but differ between individuals, we identified four longitudinally stable immunotypes that distinguished the individuals in this cohort. We characterized these immunotypes using whole blood RNA sequencing, Olink proteomic profiling, and whole blood ex vivo stimulation.