Verification of differentially expressed proteins by Western blot analysis and ROS changes in response to miR-153-3p and miR-205-5p.

Western blot analysis showing effect of miR-153-3p mimic on (A) PRDX2 and HMGB1 levels (B) effect of miR-153-3p antagomir on Cfl1 levels, (C) effect of miR-205-5p mimic on PRDX2, NACA and Cfl1 levels, (D) effect of miR-205-5p antagomir on NACA and Cfl1 levels. (E) Quantification of ROS levels in SH-SY5Y cells by modulating miR-153-3p and miR-205-5p levels. Percentage change in DCF fluorescence compared to control is shown. (F) Proposed pathway for ROS reduction due to miR-153-3p and miR-205-5p by regulation of PRDX2. Error bars indicate SEM (n = 3); *, p < 0.05; **, p < 0.01, ***; p < 0.001.