Genome-wide maps of m6a state in RA FLSs with or without ALKBH5 knockdown.

To investigate how ALKBH5 modulates RA FLSs functions, we conducted m6A sequencing (MeRIP-seq) of RA FLSs. Consistent with previous studies, in both scramble controls and ALKBH5 knockdown cells, m6A modifications were highly enriched within the GGAC motif. m6A peaks are located mainly in the protein-coding region (CDS) and 3’ untranslated region (3’ UTR) of mRNA transcripts Input group and IP group cantain three biological replicates, respectively, from human RA FLSs infected with scramble or ALKBH5 shRNA lentivirus.