Lectin-based staining of surface carbohydrate molecules on LESCs.

The LESCs surface contained high amount of sialic acid, N-acetylglucoseamine and galactose molecules. Just around half of the cells contained mannose and two thirds contained fucose molecules, showing subpopulations within the LESC cell culture. These carbohydrate molecules determine the ECM-binding and immunological properties of the cells. WGA: Wheat germ agglutinin (Triticum vulgaris), STL: Potatoe lectin (Solanum tuberosum), DSL: Datura stramonium lectin (Datura stramonium), ECL: Erythrina cristagalli lectin (Erythrina cristagalli), LEL: Tomato lectin (Lycopersicon esculentum), GSL II: Griffonia (Bandeiraea) simplicifolia lectin II (Griffonia simplicifolia), ConA: Concanavalin A (Canavalia ensiformis), RCA: Ricinus communis Agglutinin (Ricinus communis), PNA: Peanut agglutinin (Arachis hypogaea), AIL: Jacalin (Artocarpus integrifolia), VVA: Hairy vetch agglutinin (Vicia villosa), DBA: Horse gram lectin (Dolichos biflorus), SBA: Soy bean agglutinin (Glycine max), UEA: Ulex europaeus agglutinin (Ulex europaeus) (Data shown are mean±S.D., n = 3).