Brucella NyxA and NyxB dimerization enhances effector function during infection

Supporting data (uncropped RAW western blots) for the article entitled: Brucella NyxA and NyxB dimerization enhances effector function during infection
Data concern two Brucella translocated effectors, NyxA and NyxB, that contribute to the late stages of intracellular multiplication. We show that 1) NyxA and NyxB directly interact; 2) monomeric forms of the Nyx effectors still interact with their host cellular target, the deSUMOylase sentrin-specific protease 3 (SENP3); 3) monomers are less able to delocalize SENP3 from the nucleoli. , , # Data from: \*Brucella \*NyxA and NyxB dimerization enhances effector function during infection

Dataset DOI: [10.5061/dryad.fxpnvx14s](10.5061/dryad.fxpnvx14s)

## Description of the data and file structure

The dataset presented corresponds to the uncropped gels and western blots used in the manuscript entitled: Study of the molecular interactions underlying the *Brucella* NyxA and NyxB effector function.

#### File: RawData.pdf

**Figure 1.** *In vitro* and *in cellullo* interaction between NyxA and NyxB.

**(A)** Pulldown using purified NyxB against His-NyxA immobilised on a Ni NTA resin or the inverse **(B)** His-NyxB immobilized. For both, an empty column was used as a control for non-specific binding. Interactions were visualised with coomassie blue stained gels. The flowthrough (FT), wash (W) and elution (E) fractions are shown for each sample. **(D)** Co-immunoprecipitation (co-IP) assay from cells expressing NyxA or NyxB tagged with HA or Myc using anti-HA-magnetic beads. The...,