FLASH-seq of human and chimpanzee single spheroid budding at different time points

We utilized FLASH-seq, a full-length bulk RNA sequencing method, to sequence single spheroids in a 96-well format by manually sorting human and chimpanzee spheroids budding at day 3 to day 6 of midgut/hindgut patterning. We would like to use this data to assess the early stages of differentiation and to compare the intestine maturity differences between human and chimpanzee.