Raw data for Figure 2C: HIDEMs and mesoangioblasts fail to induce T cell proliferation in vitro

CFSE labelled PBMCs were stimulated with anti CD3/CD28 beads (PBMC+B) as a positive control. HIDEMs and mesoangioblasts were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h. Non-stimulated or cytokine stimulated HIDEMs/mesoangioblasts (ratio 1:4) were then co-cultured with PBMC for 6 days. CD3+ CFSE labelled 7AAD- cells were enumerated using flow cytometry and counting beads. Experiments were carried out in duplicates. n=4.

CFSE 标记的 PBMC 细胞在阳性对照实验中被抗 CD3/CD28 球珠 (PBMC+B) 激活。HIDEMs 和间充质血管祖细胞随后被 IFN-γ、TNF-α 或 IL-1β (20ng/ml) 激活处理 24 小时。未经刺激或经细胞因子刺激的 HIDEMs/间充质血管祖细胞（比例 1:4）随后与 PBMC 共同培养 6 天。使用流式细胞术和计数球珠对 CD3+ CFSE 标记的 7AAD- 细胞进行计数。实验重复进行，n=4。