Human Astrovirus CRISPR screen in Caco2 cells
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https://www.ncbi.nlm.nih.gov/sra/ERP156582
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资源简介：
Though astroviruses are important causes of disease, much of their biology, including cell surface receptors, remains unknown. We used a genome-wide CRISPR knockout library in Caco2 cells to screen for genes important in astrovirus replication. Caco2 CRISPR library cells were infected with human astrovirus 1 and sorted by flow cytometry to identify cells that were not permissive for astrovirus infection. Genomic DNA was extracted and CRISPR guides identified by PCR
创建时间：
2024-07-23
