Polymerase chain reaction mixtures and amplification conditions.
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1Symbols used: “ = seconds, ‘ = minutes.2Two separate reactions were carried out with 2 different reverse primers (CYA781Ra & CYA781Rb) according to [48]. After amplification, samples were pooled, mixed and loaded on the DGGE gel.3A final extension of 30 minutes at 95°C was applied in order to prevent band duplication according to [53].4en step touch down with annealing temperature decreasing −0.5°C per cycle.5Polymerases used: GE = GE Healthcare Taq polymerase, HS = Qiagen HotStar Taq polymerase.
创建时间：
2015-12-02
