Dtx3L and Androgen Signaling in Prostate Cancer

Gene expression in VCaP prostate cancer cells treated with or without androgen was analyzed. VCaP cells containing a dox-inducible shRNA against Dtx3L were compared with and without dox induction (Dtx3L knockdown), in the presence or absence of androgen. Overall design: RNA was extracted from VCaP cells with a Dox-inducible Dtx3L shRNA, either untreated or treated with androgen for 24 hours (2 nM, R1881). Two conditions were sequenced with 3 replicates for each condition. Data from dox-treated (Dtx3L knockdown) VCaP cells (+/- R1881) was analyzed together with data from control (no dox) VCaP cells (+/- R1881).