Light beads microscopy recordings of up to one million neurons across mouse dorsal cortex during spontaneous behavior

Example datasets and source code accompanying the article:

Manley, J., Lu, S., Barber, K., Demas, J., Kim, H., Meyer, D., Martínez Traub, F., & Vaziri, A. (2024). Simultaneous, cortex-wide dynamics of up to 1 million nuerons reveal unbounded scaling of dimensionality with neuron number. Neuron. https://doi.org/10.1016/j.neuron.2024.02.011.

Provided are example recordings of neuronal calcium dynamics across mouse cortex, captured utilizing Light Beads Microscopy (as described in Demas et al. 2021). All behavior and neural activity are spontaneous, i.e. the mouse was kept in the dark and not presented with any experimental stimuli. Neural activity was extracted using the CaImAn-based pipeline provided at https://github.com/vazirilab/MAxiMuM_processing_tools.

The following software packages are provided in .zip files in their state at the time of publication. However, it is highly recommended to utilize the repositories at https://github.com/vazirilab in case of future updates:

- scaling_analysis.zip

- MAxiMuM_processing_tools.zip

- Treadmill_control.zip

See README.md for additional details.