Relative conformational stability and immunospecificity of PrP<sup>Sc</sup> in the brains of MDR<sup>0/0</sup> mice after inoculation with untreated and MDR<sup>0/0</sup>[0>] homogenates.
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At the endpoint of disease, brain homogenates were exposed to the indicated concentrations of GdnHCl for 1 h, then to 50 µg/mL of PK for 1 h at 37°C. The levels of protease-resistant PrPSc were quantitatively measured by ELISA and normalized to 0 M GdnHCl. CDI measurements are the ratio of ELISA signals from PTA-precipitated native and denatured PrPSc as described inMaterials and Methods. Values are mean percentages ± standard error (n = 3).
创建时间：
2013-02-21
