Toxicogenomics studies reveal common and specific pathways in pulmonary, hepatic, and neuronal cell models. [SH-SY5H]

To unravel possible mechanisms of Cd tissue-specificity, and to further deepen the understanding of cadmium mechanisms, we have compared transcriptomics data from cell models representative of three major Cd targets: pulmonary (A549), hepatic (HepG2), and neuronal (SH-SY-5Y) cells. Results from KEGG analysis show that only one specific pathway is dysregulated in a significant way in all cell models: the mineral absorption pathway. Human neuroblastoma cells (SH-SY5Y) were exposed to non-cytotoxic cadmium concentrations (CdCl2) 10 or 20 µM for 24h. Two biological replicates of controls (cells grown in complete culture medium) were performd and 3 biologial replicates for each cadmium concentration used.