File S1 - Preparation of Artificial Plasma Membrane Mimicking Vesicles with Lipid Asymmetry

Figure S1, Lack of cholesterol extraction by HPαCD. Figure S2, HPαCD-induced exchange efficiency is dependent on the ratio of donor/acceptor vesicle concentration. Figure S3, Comparison of vesicle size before and after HPαCD-induced lipid exchange. Figure S4, Schematic representation of protocol for measuring contamination of asymmetric vesicles by donor vesicles using C6-NBD-PC. Figure S5, HP-TLC chromatograms of asymmetric vesicle preparations. Figure S6, Assay of lipid asymmetry detected by binding of hydrophobic helix pL4A18. Figure S7, TNBS labeling of POPE in exchange (asymmetric) vesicles outer leaflet. Figure S8, Stability of exchange (asymmetric) vesicles. Table S1, Measurement of contamination from “donor” vesicles by NBD reduction. Table S2, Lipid composition in exchange vesicles. (DOCX)