Supplementary information S2 movies

Supplementary Information 2. Representative movies of the phase contrast and epifluorescence (reporting sfGFP(Sp) expressed from the TnJM1 transposon inserted into the indicated genes) channels of reconstructed mutants grown on MOPS agarose pads. Due to differences in fluorescence expression among the isolated clones, image brightness was adjusted for each mutant separately and fluorescence intensity can therefore not be compared between videos.

补充信息2. 重建突变体在MOPS琼脂垫上生长的相衬和共聚焦（报告sfGFP(Sp)从插入指定基因的TnJM1转座子中表达）通道的代表性电影。由于分离克隆之间荧光表达存在差异，因此对每个突变体分别调整了图像亮度，因此荧光强度无法在视频之间进行比较。