Single cell SMART RNAseq on WT and TKO E14 EpiLCs

We investigate how DNA methylation absence impact transcriptionnal regulation during epiblast differentiation. More specifically, how synchronous is the naïve to primed transitioning in TKO cells compared to WT, and if there is a "germline-competent" TKO subpopulation of EpiLCs. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation WT and TKO at D0, D1, D2 and D4. We next performed single-cell SMARTseq2 RNA sequencing. Overall design: Comparison of Single Cell RNA sequencing results (SMARTseq2 protocol) results of WT and TKO during EpiLC differentiation (D0, D1, D2, D4)