Additional file 2 of Application of dried blood spot sample pooling strategies for Plasmodium 18S rRNA biomarker testing to facilitate identification of infected persons in large-scale epidemiological studies

Additional file 2: Text S2. Primers/probes concentrations used for the UW qRT-PCR assay and DNA sequences. Plasmodium falciparum qRT-PCR employed 0.4 µM of PfDDT1451F21 (5’-GCGAGTACACTATATTCTTAT-3’), 0.4 µM of PfDDT1562R21 (5’-ATTATTAGTAGAACAGGGAAA-3’) and 0.1 µM of Pf probe (5'-[6-FAM]-ATTTATTCAGTAATCAAATTAGGAT-3'); for Pan Plasmodium qRT-PCR, they were 0.2 µM of PanDDT1043F19 (5’-AAAGTTAAGGGAGTGAAGA-3’), 0.2 µM of PanDDT1197R22 (5’-AAGACTTTGATTTCTCATAAGG -3’) and 0.1 µM of Pan probe (5'-[CAL Fluor Orange 560]-ACCGTCGTAATCTTAACCATAAACTATGCCGACTAG-3'); for TBP RT-PCR, they were 0.1 µM of TBP forward primer (5’-GATAAGAGAGCCACGAACCAC-3’), 0.1 µM of TBP reverse primer (5’- CAAGAACTTAGCTGGAAAACCC-3’) and 0.1 µM of TBP probe (5'-[Quasar 670]- CACAGGAGCCAAGAGTGAAGAACAGT-3').