Effects of selection for ethanol preference on gene expression (RNA-Seq) in the nucleus accumbens shell of HS-CC mice. Mus musculus

Nucleus accumbens shell gene expression was assessed by RNA-Seq to determine effects of bidirectional selection for ethanol preference from a highly genetically diverse founder population. We analyzed the data with both standard methodologies to detect differential expression, and coexpression network changes, and with novel methodologies to detect alternative exon usage, and coordinated splicing pattern changes due to selection. Overall design: 48 samples of HSCC animals selected for high and low ethanol preference were analyzed (selection generation 4): 24 females (12 High, 12 Low) 24 males (12 High, 12 Low).