RT-PCR validation^a of the array expression values with 21 genes^b on Ch5b in Sor55^c.

aSee Fig. S1 for examples of semi-quantitative RT-PCR gels. bSee Table S3 for all 30 genes, Table S1 for gene systematic names. For Northern blot analyses see the Results section and Table S4. cNote that also shown are RT-PCR estimates of the expression level of several corresponding genes on Ch5a in Sor60. dNote that CTA24 is duplicated on Ch5b in Sor55, Table S2 and legend to Fig. 5, and is serving here, as a control for the expression from two copies of gene. The other genes have aCGH ratios Sor55/3153A ranging from 0.51 to 0.64 with two ratios equaling to 0.65 and 0.68. Most probably, all those genes possess a single copy. Expression changes were calculated, as mean ratios Sor55/3153A for Ch5b or Sor60/3153A for Ch5a. Data were obtained from independent experiments.