Genome wide DNA methylation sequencing by XmaI-RRBS resolves breast cancer epigenetic heterogeneity and suggests novel perspectives for personalized therapy

Aim: To provide a breast cancer (BC) methylotype classification by genome-wide CpG islands bisulfite DNA sequencing. Results: Overall, 6 distinct BC methylotypes were identified. Of these, BC cell lines constitute a separate group extremely highly methylated at the CpG islands. In turn, primary BC samples segregate into two major subtypes, highly and moderately methylated. Overall design: XmaI-RRBS DNA methylation sequencing method was used to profile DNA methylation of 111 BC samples and 6 normal breast samples.