UTR annotations for TriTrypDB trypanosomatid parasite genomes

This dataset provides 5′ and 3′ untranslated region (UTR) annotations and Open Reading Frames (ORFs) corrected based on UTRs for 47 trypanosomatid genomes hosted on TriTrypDB (version 68). Trypanosomatids, including human pathogens such as Leishmania and Trypanosoma species, utilise polycistronic transcription where mature mRNAs are generated via trans-splicing and polyadenylation. These data were generated using the slapquant software suite, which identifies spliced leader acceptor sites (SLAS) and polyadenylation sites (PAS) from short-read RNA-seq data using a clipped-alignment strategy, then uses these to define UTRs and potential ORF corrections. Files Included: Annotated GFF Files: Genome-wide functional annotation files for 47 species/strains containing identified SLAS, PAS, and resulting 5′ and 3′ UTRs (files containing the _slaputrs filename suffix). Corrected CDS/ORFs Files: Corrected protein-coding sequences (CDS) where trans-splicing evidence (SLAS positions) suggested a more likely upstream or downstream start codon than the original annotation (files containing the -fixedorfs filename suffix). Result files are named after the corresponding genome identifier on TriTrypDB, and should be used with the corresponding genome sequence fasta file from TriTrypDB version 68. Software Availability: The tools used to generate these files are available at: https://github.com/Wheeler-Lab/slapquant.