Jennifer Bestman, Holly Cline (2011) CIL:37076, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #3 (hour 4) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The cell was imaged at a 2 hour intervals over 8 hours and shows the development and morphological plasticity of the dendritic arbor and axon over time.

The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

此为来自无色斑马鱼蝾螈中枢神经系统（CNS）视顶盖中发育中的神经元第3张图像（第4小时）。该细胞在2小时间隔内连续8小时进行成像，展现了树突丛和轴突随时间推移的发育与形态可塑性。在获取图像前约24小时，通过体内电穿孔法将编码增强型绿色荧光蛋白（eGFP）的质粒DNA转染至该细胞。将完整的活体蝾螈进行麻醉，置于玻璃盖片下，通过定制构建的双光子显微镜直接透过其透明皮肤进行成像。