Stephane Brunet, Julien Dumont, Karen W. Lee, Kazuhisha Kinoshita, Pascale Hikal, Oliver J. Gruss, Bernard Maro, Marie-Helene Verlhac (2011) CIL:35432, Mus musculus, oocyte. CIL. Dataset

This 3 panel image displays meiosis in a mouse oocyte triple labelled for microtubules (panel 1 and green), phosphorylated-Transforming Acidic Coiled-Coil protein (TACC3; panel 2 and red), and nucleic acids (blue). This oocyte was injected with siTPX2-5h, a doublestranded siRNA against TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2)and rescued with the coinjection of YFP-TPX2DN, a truncated version of TPX2, which lacks the 118 first amino acids.

Figure 7a, fourth row. doi:10.1371/journal.pone.0003338.g007

该三联图像展示了小鼠卵母细胞中的减数分裂过程，该卵母细胞经微管（面板1及绿色）、磷酸化酸性卷曲螺旋蛋白（TACC3；面板2及红色）和核酸（蓝色）三重标记。此卵母细胞被注射了双链siRNA siTPX2-5h，针对TPX2（针对非洲爪蟾肌球蛋白样蛋白，xklp2的靶蛋白）进行靶向，并通过与YFP-TPX2DN的共同注射得到挽救，YFP-TPX2DN是TPX2的截断版本，缺失了前118个氨基酸。