Selection-free CRISPR-Cas9 editing protocol for distant Dictyostelid species

Folder "A" contains all raw nanopore reads Folder "B" contains the annotated de novo D. discoideum AX2 act5::mCherry genome assembly (i.e., reference genome), Folder "C" contains the alignment of all reads against our reference genome (i.e., original BAM files). Folder "D" contains the alignment of all reads containing mCherry against our reference genome. Folder "E" contains the alignment of all reads containing 37nt donor oligo against our reference genome. Folder "Files S1-S6, Data S1", all supplementary data files associated with protocol and manuscript