Improvement of hepatocellular carcinoma using hepatocyte-derived liver progenitor-like cells (HepLPCs): therapeutic, proliferation arrest, malignant biological behavior changes,signal pathway screening

We employed a co-culture experiment and found that human hepatocyte-derived liver progenitor-like cells (HepLPCs) significantly inhibited the proliferation of hepatocellular carcinoma (HCC) in a specific manner and altered their malignant biological behavior without affecting cell aging, autophagy, apoptosis and so on. To evaluate the mechanism of action, HepG2 and Hep3B after co-cultured with HepLPCs gene profiling were performed, and we observed, in addition to proliferative blockade induction, that cascade events originated from mitochondrial dysfunction. Overall design: Global expression analysis of RNA sequencing data of expression levels of HepG2 and Hep3B in the presence or absence of hepatocytes derived liver progenitor-like cells (HepLPCs), and the global expression of HepG2 and Hep3B genes after combined inhibition of Notch1 and STAT3.