Dating the origin and spread of plastids and chromatophores

Photosynthetic eukaryotes have shaped the Earth's biosphere by producing oxygen and converting light into organic compounds in specialized organelles called plastids. Plastids evolved from free-living cyanobacteria ingested by heterotrophic unicellular eukaryotes. Two such independent engulfment processes, called cyanobacterial endosymbioses, have been reported so far. The first gave rise to primary plastids and three Archaeplastida lineages: glaucophytes, red algae and green algae with land plants, whereas the second resulted in chromatophores in the rhizarian amoeba Paulinella. Importantly, archaeplastidans donated their plastids to many protist groups, thereby further spreading photosynthesis across the tree of life. To reveal the complex plastid evolution, we performed comprehensive phylogenetic and multi-clock analyses based on new fossil calibration points and the greatest number yet of plastid-encoded proteins from 108 taxa, representing a large diversity of photosynthetic organi..., Please see the README document (\"README.txt \") and the SUPPLEMENTARY MATERIALS file (“Supplementary_Information.docx”)., Please see the README document (\"README.txt \") and the SUPPLEMENTARY MATERIALS file (“Supplementary_Information.docx”).