Chisholm Lake sedimentary unprocessed fish metabarcoding DNA sequences

We sequenced amplicons targeting a segment of the 12S ribosomal DNA gene in vertebrates from DNA pools isolated from sections of a sediment core from Chisholm Lake in interior Alaska. The primary aim of this sequencing effort was to add to our understanding of the evolution of fish faunas in Beringian regions impacted by glacial cycles. This dataset consists of unprocessed output from amplicon paired end short read sequencing on an Illumina sequencing by synthesis platform. Analyses of these sequences are being incorporated into a broader effort to examine post-glacial Beringian biotas and ecologies.

Methods
DNA was isolated from sections from a lake bottom sediment core. DNA isolates were used as PCR templates using the reagents described in the MiFish metabarcoding protocols of Miya et al. (2015). Amplicons were then used to prepare libraries suitable for sequencing on Illumina platforms. All libraries generated from core sections and section replicates were uniquely indexed then pooled together and sequenced on a MiSeq instrument.