Steps of the High-Throughput PI5P4Kα Assay.
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Step Notes.1. Dispensed into white solid-bottom 1536-well MB Greiner plates with a Flying Reagent Dispenser (FRD). Reagent is kept on ice. Final concentration is 10 nM PI5P4Kα, 75 µM PI5P and 150 µM DPPS.2. Plates centrifuged at 300×g for 10 seconds.3. DMSO compound solutions transferred with a Kalypsys pintool.4. Dispensed with a FRD. Final concentration is 5 µM ATP.5. Plates incubated at room temperature.6. Dispensed with a FRD.7. Plates incubated at room temperature.8. Dispensed with a FRD.9. Plates incubated at room temperature.10. Luminescence was measured with a ViewLux CCD Imager with a 20 second exposure time.
创建时间：
2015-12-02
