The mapping values and averages for three sheep heart transcriptome experiments were built into a de novo assembly using Trinity and annotated with Trinotate. Reads were mapped to the assembly using S
*Q20 percentage: Percentage of nucleotide error rate under 0.01. **N: Uncertain base in the output sequencing data. ***N50: Median length of all contigs or unigenes.
This is a workshop for TraRECo, a de novo transcriptome assembler with read error correction, developed by S. Yoon.The workshop include MATLAB scripts to implement the assembly and configuration file