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ChIP-seq of H2A.Z and its histone chaperones during the cell cycle. ChIP-seq of H2A.Z and its histone chaperones during the cell cycle

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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB52203
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The aim of this study was to uncover cell cycle dependent chromatin binding of H2A.Z and its histone chaperones. U2OS cells were stably transfected with Twin-Strep-tagged H2A.Z, ANP32e, or YL1 constructs, and WT cells were used as a negative/background control. U2OS cells were synchronised in G1 or G2-M phase using hydroxyurea or nocodazole, respectively, and protein-DNA complexes were isolated by affinity purification.
创建时间:
2023-01-31
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