Metatranscriptome of lab-reared Spodoptera frugiperda larvae guts

The purpose of this study is to perform a comprehensive analysis which integrates Spodoptera frugiperda gene expression data with environmental information and taxa associated with S. frugiperda larvae guts. For this, total RNA extracted from laboratory reared S. frugiperda larvae guts, originally collected from a maize (Zea mays) field in Tucumán province (Argentina), was submitted to sequence-independent amplification and high-throughput pyrosequencing. Due to the fact that total RNA was amplified using a modified sequence-independent amplification protocol and, for this, first-strand reverse transcription was initiated with a random octamer linked to a specific primer sequence, an unbiased random collection of transcripts was obtained for each sample.