Structure an LPyV VP1 carbohydrate receptor complex.

A. Overview of the LPyV VP1 structure in complex with 3SL. A LPyV VP1 pentamer is shown in cartoon representation, with one monomer highlighted in green. The oligosaccharide ligand is drawn in stick representation and colored by element, with carbons in orange, nitrogens in blue and oxygens in red. The calcium ion bound to the free LPyV VP1 pentamer is shown as a cyan sphere, with LPyV residues contacting it shown in stick representation. Residues 28–38 at the N-terminus are involved in non-native crystal contacts and are not shown here for clarity. B. and C. Close-up views of the 3SL (B) and 3SLN (C) binding sites. LPyV VP1 is drawn as in (A). The oligosaccharides are shown in stick representation, with 3SL in orange and 3SLN in yellow. Composite annealed omit difference density maps are shown contoured at 2.5 σ with a radius of 3.5 Å around the respective oligosaccharides.