Adiponectin rescues synaptic plasticity in the dentate gyrus of a mouse model of fragile X syndrome

Fragile X Syndrome (FXS) is the most common inherited cause of intellectual disability and is the leading known single-gene cause of autism spectrum disorder. FXS patients display varied behavioural deficits that include mild to severe cognitive impairments in addition to mood disorders. Currently, there is no cure for this condition, however, there is an emerging focus on therapies that inhibit mTOR-dependent protein synthesis due to the clinical effectiveness of metformin for alleviating some behavioural symptoms in FXS. Adiponectin (APN) is a neurohormone that is released by adipocytes and provides an alternative means to inhibit mTOR activation in the brain. In these studies, we show that Fmr1 KO mice, like FXS patients, show reduced levels of circulating APN, and that both LTP and LTD in the DG (dentate gyrus) are impaired. Brief (20 min) incubation of hippocampal slices in APN (50 nM) was able to rescue both LTP and LTD in the DG and increased both the surface expression and phosp..., Slices were perfused with ACSF (30 °C) at a rate of approximately 2 ml/min and field excitatory postsynaptic potentials (fEPSPs) recorded using an Axon MultiClamp 700B amplifier connected to a PC running Clampex 10.2 software (Molecular Devices, CA, USA). An upright Olympus BX50WI microscope was used to assist with visually placing stimulating and recording electrodes in the middle of the molecular lay of the DG as previously described. fEPSPs were elicited by delivering a 120 μs (10–40 μA) current pulse every 15s to the medial perforant path (MPP) via a concentric bipolar stimulating electrode (FHC, Bowdoin, ME). A borosilicate glass recording electrode (1 MΩ) was filled with ACSF and visually placed about 200 μm from the stimulating electrode. A stable baseline was recorded for at least 15 min using a stimulation magnitude that elicit a response of 50% of the maximum amplitude obtained for synaptic potentiation, and 70% for synaptic depression experiments. Following this, a paired-pul..., , # Adiponectin rescues synaptic plasticity in the dentate gyrus of a mouse model of fragile X syndrome

[https://doi.org/10.5061/dryad.rxwdbrvh4](https://doi.org/10.5061/dryad.rxwdbrvh4)

## Description of the data and file structure

An excel spreadsheet is provided that consists of 4 sheets. The first presents the individual slice data from each animal in a series of columns. Columns are grouped according to experimental group. Page 1 is specific to LTP data, while Page 2 is specific to LTD data. On page 3, the input/output data for each slice is presented in a series of boxes/columns for each experimental group. On page 4, the paired-pulse data for each slice is presented in a series of boxes/columns for each experimental group.

## Sharing/Access information

Additional information can be obtained by directly contacting the authors. 

## Code/Software

All data was analyzed using PClamp software, with slope values exported into Excel. Statistical analyses were performed using GraphPa...