IFN-lambda gut restitution scRNA-seq — WT sample (20230601_10X_RNAseq_WT): R session images (selected clustering resolutions)

Type III interferons induce pyroptosis in gut epithelial cells and impair mucosal repair To investigate the transcriptional program within the intestinal epithelial cells (IECs) controlling the impact of type III interferons (IFN-λ) on intestinal recovery, we performed a single-cell RNA sequencing (scRNA-seq) on murine IECs following irradiation. Mice deficient for the IFN-λ receptor in IECs and wild-type mice underwent targeted irradiation. 96h after irradiation, IECs were isolated by fluorescence-activated cell sorting (FACS) based on the expression of EPCAM and analyzed using scRNA-seq. This record Single archive rdata_WT.tar, 6 files, 40.4 GB. R session images (.RDATA) saved at the end of each analysis step. Only the clustering resolutions that are actually consumed by a downstream step are included; the rendered reports for all explored resolutions remain in the corresponding core record. How to use these records Every archive stores paths relative to the same root, 002_IFNL_Recovery_scRNAseq/. Download the archives you need and extract them all into one empty directory: tar xf *.tar Each file lands in its correct place in the project tree. Taking only the core records gives a complete but lightweight tree; adding rdata or bam records fills it in without overwriting anything.