Expression data from activated bone marrow derived macrophages from control and BRD4 conditional KO mice. Expression data from activated bone marrow derived macrophages from control and BRD4 conditional KO mice

Macrophages are cells belongs to innate immune system, which response to pathogen by the production of inflammatory proteins those that are effective in both combating pathogen and wound healing. Using small molecule inhibitors it has been shown that many of the inflammatory response genes were under control of BET proteins. We used LysM-Cre to conditionally delete floxed BRD4 in bone marrow derived macrophages and monitored it's effect on inflammatory gene expression Overall design: Bone marrow derived macrophages were treated with either LPS, Interferon gamma and IL-4 alone or with LPS with a pre treatment of interferon gamma for RNA extraction and hybridization on Affymetrix microarray. Samples from untreated cells were used as control. To compare differential gene expression samples were prepared from wild type and BRD4 deleted macrophages. Biological duplicates were used for each treatment condition