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Whole genome sequencing of human HapMap individual NA12877 using the Illumina HiSeq 2000 and paired 100 base reads

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NIAID Data Ecosystem2026-03-07 收录
下载链接:
https://www.ncbi.nlm.nih.gov/bioproject/PRJEB2889
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We have generated paired-end sequence data covering the genome of an CEPH male individual to a sequence depth of more than 30-fold using the Illumina HiSeq 2000. This individual is a member of the population samples described in the PhaseI and PhaseII HapMap Projects and is from the CEPH/UTAH pedigree 1463 (abbreviation: CEPH). The DNA identifier for this individual is NA12877. We obtained the DNA sample NA12877 from The Coriell Institute for Medical Research. Starting with 1ug of DNA, and following random fragmentation, we generated a PCR-Free sequencing library with a median insert size of ~300 bp. 100 base sequence reads were generated from both ends of these templates using the Illumina HiSeq 2000. We carried out purity-filtering (PF) to remove mixed reads, where two or more different template molecules are close enough on the surface of the flow-cell to form a mixed or overlapping cluster. No other filtering of the data has been carried out prior to submission. We have also submitted equivalent sequence data for the mother and one of the son's of family 1463 (NA12878 and NA12882).
创建时间:
2012-02-17
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