Table_2_Integrated Analysis of Metabolome and Transcriptome Data for Uncovering Flavonoid Components of Zanthoxylum bungeanum Maxim. Leaves Under Drought Stress.XLS

Zanthoxylum bungeanum Maxim. leaves (ZBLs) are rich in flavonoids and have become popular in nutrition, foods and medicine. However, the flavonoid components in ZBLs and the mechanism of flavonoid biosynthesis under drought stress have received little attention. Here, we performed an integrative analysis of the metabolome and transcriptome of ZBLs from HJ (Z. bungeanum cv. “Hanjiao”) and FJ (Z. bungeanum cv. “Fengjiao”) at four drought stages. A total of 231 individual flavonoids divided into nine classes were identified and flavones and flavonols were considered the most abundant flavonoid components in ZBLs. The total flavonoid content of ZBLs was higher in FJ; it increased in FJ under drought stress but decreased in HJ. Nine-quadrant analysis identified five and eight differentially abundant flavonoids in FJ and HJ leaves, respectively, under drought stress. Weighted gene correlation network analysis (WGCNA) identified nine structural genes and eight transcription factor genes involved in the regulation of flavonoid biosynthesis. Moreover, qRT-PCR results verified the accuracy of the transcriptome data and the reliability of the candidate genes. Taken together, our results reveal the flavonoid components of ZBLs and document changes in flavonoid metabolism under drought stress, providing valuable information for nutrition value and food utilization of ZBLs.

Zanthoxylum bungeanum Maxim.（香橼木）的叶子富含黄酮类化合物，并在营养、食品及医药领域日益受到青睐。然而，关于香橼木叶中黄酮类成分及其在干旱胁迫下黄酮生物合成机制的探讨尚显不足。本研究对HJ（香橼木品种“寒角”）和FJ（香橼木品种“丰角”）在四个干旱阶段的代谢组学和转录组学进行了综合分析。共鉴定出231种黄酮类化合物，分为九类，其中黄酮类和黄酮醇类被认为是香橼木叶中最为丰富的黄酮成分。FJ中黄酮类总含量高于HJ；在干旱胁迫下，FJ的黄酮含量增加，而HJ的黄酮含量则降低。九象限分析识别出在干旱胁迫下FJ和HJ叶中分别有五种和八种差异丰度的黄酮类化合物。加权基因相关网络分析（WGCNA）确定了九个结构基因和八个转录因子基因，它们参与调控黄酮生物合成。此外，实时荧光定量PCR（qRT-PCR）结果验证了转录组数据的准确性和候选基因的可靠性。综合来看，本研究揭示了香橼木叶中黄酮类成分，并记录了干旱胁迫下黄酮代谢的变化，为香橼木叶的营养价值和食品利用提供了宝贵信息。