Replication Data of the Effects of Green Tea and/or Licorice Aqueous Extracts on Lipids Profile of Dimethylnitrosamine-Intoxicated Male Albino Rats.

A) Body weight gain and relative liver weight 1. Effect of the tested materials on the % of body weight gain Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P < 0.001). Green tea, licorice or the mixture of both, all caused significant decreases in the % of body weight gain relative to normal control by 74.48 %, 78.48 % or 67.09 % respectively. The increase in the % of the body weight gain caused by DMN was 93.67 % relative to control. Licorice administration to DMN-treated rats led to more increase in body weight gain by 81.70 % while green tea or the mixture of those extracts caused marked reduction relative to DMN-administered rats by 96.08 % or 126.80 % as shown in table 1. 2. Effect of the tested materials on the % of liver/body weight ratio Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P < 0.01). Results indicated that green tea, licorice, DMN or the mixture exerted no significant change relative to normal control. The DMN groups administered with green tea, licorice or the mixture showed no significant changes when compared with DMN control group as shown in table 1. B) Biochemical studies 1. Effect of the tested materials on plasma level of total cholesterol Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P < 0.001). The mixture of the extracts caused a decrease but insignificant when it was compared with normal control while licorice, DMN or green tea caused significant decreases 8.18 %, 4.67 % or 4.67 % respectively. The administration of green tea to DMN-intoxicated rats caused a significant decrease by 5.21 % when compared with DMN control rats, while the mixture or licorice caused significant increase by 8.78 % or 6.84 % respectively as shown in table 2. 2. Effect of the tested materials on plasma level of LDL-cholesterol Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P < 0.001). Green tea, licorice, DMN or the mixture of extracts induced significant decreases in plasma level of LDL-cholesterol relative to normal control by 21.45 %, 54.18 %, 30.55 or 21.81 % respectively. Green tea enhanced the effect of DMN by producing more decrease (-33.50 %). On the other hand, licorice or the mixture antagonized the effect of DMN by increasing the level by 32.98 % or 46.01 % respectively as compared to DMN control group as shown in table 2. 3. Effect of the tested materials on plasma level of HDL-cholesterol Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P < 0.01). As shown in table 2, green tea or licorice exerted no significant changes in the level of plasma HDL-cholesterol relative to normal control, on contrary to that, the mixture of extracts or DMN produced a significant increase by 8.61 % or 5.04 % respectively. Green tea, licorice or the mixture exerted no significant effect on the rising effect of DMN. 4. Effect of the tested materials on plasma level of total-cholesterol/HDL-cholesterol ratio Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P < 0.001). Green tea exerted an insignificant change in that ratio relative to normal control, while licorice, DMN or the mixture caused a significant decrease by 11.39 %, 9.90 % or 8.91 % respectively. Green tea synergisted the lowering effect of DMN by decreasing significantly that ratio by 7.69%, while the mixture attenuated the DMN effect by increasing that ratio significantly by 9.34 % when they were compared with DMN control group. On the other hand, licorice intake to DMN-administered rats caused an insignificant increase as shown in table 3. 5. Effect of the tested materials on plasma level of triglyceride (TG) Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P < 0.001). As shown in table 3, licorice, DMN, green tea or the mixture increased the plasma level triglyceride significantly relative to normal control by 20.03 %, 4.41 %, 3.41 % or 3.33 %. Green tea, licorice or the mixture intake to DMN-administered animals exerted no significant change when compared to the corresponding DMN control group.