Sequencing of Red drum microbiota

This study aims to know the evolution of the microbiota of Red drum (Sciaenops ocellatus) filets using the Illumina MiSeq platform to sequencing the 16S rRNA gene V3-V4 hypervariable region. Two storage conditions were analyzed: vacuum packaging (code sample = VP); modified atmosphere (50% CO2, 50% N2) (code sample = MAP). VP and MAP samples were stored at 4°C. Samples were analyzed before the packaging (fresh tuna) (code sample = D0) and after eight, fifteen, twenty-two and twenty-nine days of storage (code sample = D8, D15, D22, D29). Sequencing data were analyzed using the Qiime 1.9.1 pipeline. Forward and reverse reads were joined using fastq-join and quality filtered using default Qiime parameters.