Identification of endogenous changes in the transcriptome of young versus old Drosophila intestinal stem cells using Next Generation RNA Sequencing
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=a188b9f50bc5fcc771c566b4bfc1391d
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The Drosophila midgut is an ideal model system to study molecular mechanisms that interfere with the intestinal stem cellsâ (ISCs) ability to function in tissue homeostasis. Due to the lack of a combination of molecular markers suitable to isolate ISCs from aged intestines, it has been a major challenge to study endogenous molecular changes of ISCs during aging. Our FACS-based approach using the esg-GAL4, UAS-GFP fly line allowed the isolation of a cell population enriched for ISCs from young and old midguts by their small size, little granularity and low GFP intensity. The isolated ISCs were subsequently used for RNA sequencing to identify endogenous changes in the transcriptome of young versus old ISCs.
提供机构：
University of Ulm
创建时间：
2022-02-20
