Genetic analyses reveal the signaling and transcriptional mechanisms of stomach stromal stem cell niches. [RNA-Seq]

We demonstrate the conserved Hh-GLI2-mediated chromatin and transcriptional regulation of both stomach and intestinal stromal stem cell niche signals. Analyses of H3K27ac marks demonstrate GLI2-mediated transcription regulation of stem cell niche signals such as Wnt ligand genes, through enhancers conserved between the stomach and intestine. Overall design: RNA-Seq of E17.5 wildtype and Sufu;Spop double knockout stomach in biological duplicates