Effect of Ythdf2 in haematopoiesis [RNA-seq]

The mRNA m6A reader YTHDF2 is overexpressed in human acute myeloid leukaemias. To understand the role of YTHDF2 in normal haematopoiesis and ageing, we performed SMART-seq on haematopoietic stem cells (HSCs) derived from young and aged (1 year old) mice. In parallel, to identify transcripts likely methylated in HSCs we performed meRIP-seq analysis of c-Kit+ cells. In young mice, two samples per mouse were taken from 4 WT vs 4 KO mice, but since replicate samples taken from each mouse behaved as biological replicates, they were treated as such. In aged mice, two samples were taken per mouse from 4 WT vs 5 KO mice, and again, because replicate samples from the same mouse acted as biological replicates, these samples were treated as such. Please note that the 'aged_WT99_HSC_B' sample has low read counts and is excluded from further analysis.