PMA +/- NFkB inhibition in adult rat cardiomyocytes
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Rat cardiomyocytes (adult female Wistar rats) were isolated and kept in culture for a few hours; NFkB inhibitors were added 1 h prior to the addition of PMA (50 nM) and then kept in culture for another 4 h (total culture time: 5 h). Control cells (CTRL) were treated with vehicles only for 5 h. NFkB inhibitors and concentrations were TPCA1 (10 µM) + NIKSMI1 (0.5 µM). The group is named NTP (NIKSMI1+TPCA1+PMA).
