<i>B. nonstop</i> annotated proteins
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Protein sequences from <i>B. nonstop</i> were additionally annotated by blastp searches (with a cut-off <i>e</i>-value of 10<sup>-20</sup>) against protein datasets of a selection of kinetoplastids available from TriTrypDB (release 62) including <i>Angomonas deanei</i>, <i>Blechomonas ayalai</i>, <i>Bodo saltans</i>, <i>Crithidia fasciculata</i>, <i>Endotrypanum monterogeii</i>, <i>Leishmania major</i> Friedlin, <i>Leishmania martiniquensis</i>, <i>Leishmania mexicana</i>, <i>Leptomonas pyrrhocoris</i>, <i>Leptomonas seymouri</i>, <i>Paratrypanosoma confusum</i>, <i>Porcisia hertigi</i>, <i>Trypanosoma brucei</i> TREU927, <i>Trypanosoma cruzi</i> CL Brener Esmeraldo-like. Proteins not retrieving any hit were then screened (with a cut-off <i>e</i>-value of 10<sup>-5</sup>) against the Swiss-Prot database (downloaded February 10<sup>th</sup>, 2023) (Bairoch & Apweiler 2000, Nucleic Acids Res). Protein domains were further annotated using InterProScan v5.55-88.0 (Jones <i>et al.</i> 2014, Bioinformatics) and the Pfam database (Finn et al. 2014, Nucleic Acid Res).
针对<i>B. nonstop</i>的蛋白质序列，本研究以期望值（e-value）阈值10<sup>-20</sup>为标准，通过BLASTp比对（blastp）进行额外注释，比对所用的数据集来自TriTrypDB（版本62）中收录的多款动基体目原生动物的蛋白质组，所涉物种包括<i>Angomonas deanei</i>、<i>Blechomonas ayalai</i>、<i>Bodo saltans</i>、<i>Crithidia fasciculata</i>、<i>Endotrypanum monterogeii</i>、<i>Leishmania major</i> Friedlin、<i>Leishmania martiniquensis</i>、<i>Leishmania mexicana</i>、<i>Leptomonas pyrrhocoris</i>、<i>Leptomonas seymouri</i>、<i>Paratrypanosoma confusum</i>、<i>Porcisia hertigi</i>、<i>Trypanosoma brucei</i> TREU927 及<i>Trypanosoma cruzi</i> CL Brener Esmeraldo-like。对于未检索到任何有效比对结果的蛋白质序列，本研究再以期望值阈值10<sup>-5</sup>为筛选标准，将其与2023年2月10日下载的Swiss-Prot数据库（Bairoch与Apweiler, 2000年, *Nucleic Acids Res*）进行比对筛选；此外，还通过InterProScan v5.55-88.0（Jones等, 2014年, *Bioinformatics*）与Pfam数据库（Finn等, 2014年, *Nucleic Acids Res*）对蛋白质结构域进行了进一步注释。
