Recipient specific T cell repertoire reconstitution in the gut following murine hematopoietic cell transplant

Graft-versus-host-disease (GVHD) is a complication of hematopoietic cell transplantation (HCT) caused by alloreactive T cells. Murine models of HCT are used to understand GVHD and T cell reconstitution in GVHD target organs, most notably the gastrointestinal (GI) tract where the disease contributes most to mortality in patients. We used T cell receptor repertoire sequencing (TCR-Seq) to measure T cell reconstitution from the donor graft (C57BL/6 H-2b) in the GI tract of different recipients across a spectrum of matching: from syngeneic (C57BL/6), to minor histocompatibility (MHC) antigen mismatch BALB.B (H-2b), to major MHC mismatched B10.BR (H-2k) and BALB/c (H-2d).