Modifications to the genomic DNA protocol of Ivanova et al. 2006 used for sponge barcoding.
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1Lysis mix: 100 mM NaCl, 50 mM Tris-HCl pH 8.0, 10 mM EDTA pH 8.0, 0.5% SDS, Proteinase K 10% v/v.2Binding buffer: 6 M GuSCN, 20 mM EDTA pH 8.0, 10 mM Tris-HCl pH 6.4, Triton X-100 4% v/v. The Binding mix is a 50% v/v solution of Binding Buffer in ethanol 96%.3Protein wash buffer is a 30% v/v solution of Binding Buffer in ethanol 96%.4Wash buffer: 50 mM NaCl, 10 mM Tris-HCl pH 7.4, 0.5 mM EDTA pH 8.0, ethanol 60%.
创建时间：
2015-12-02
